basic fmri system Search Results


recombinant human basic fibroblast growth factor  (R&D Systems)
95
R&D Systems recombinant human basic fibroblast growth factor
Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS <t>fibroblast</t> cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using <t>recombinant</t> FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.
Recombinant Human Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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basic fmri system  (NordicNeuroLab AS)
90
NordicNeuroLab AS basic fmri system
Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS <t>fibroblast</t> cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using <t>recombinant</t> FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.
Basic Fmri System, supplied by NordicNeuroLab AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basic fmri system/product/NordicNeuroLab AS
Average 90 stars, based on 1 article reviews
basic fmri system - by Bioz Stars, 2026-06
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trios 3-t scanner  (Siemens AG)
90
Siemens AG trios 3-t scanner
Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS <t>fibroblast</t> cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using <t>recombinant</t> FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.
Trios 3 T Scanner, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trios 3-t scanner/product/Siemens AG
Average 90 stars, based on 1 article reviews
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fmri signal  (Pfeuffer GmbH)
90
Pfeuffer GmbH fmri signal
Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS <t>fibroblast</t> cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using <t>recombinant</t> FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.
Fmri Signal, supplied by Pfeuffer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fmri signal/product/Pfeuffer GmbH
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1.5-t mri vantage titan  (TOSHIBA Medical)
90
TOSHIBA Medical 1.5-t mri vantage titan
Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS <t>fibroblast</t> cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using <t>recombinant</t> FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.
1.5 T Mri Vantage Titan, supplied by TOSHIBA Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1.5-t mri vantage titan/product/TOSHIBA Medical
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1.5-t mri vantage titan - by Bioz Stars, 2026-06
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Image Search Results


Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS fibroblast cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using recombinant FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.

Journal: Stem cells translational medicine

Article Title: High-Throughput Screening to Identify Compounds That Increase Fragile X Mental Retardation Protein Expression in Neural Stem Cells Differentiated From Fragile X Syndrome Patient-Derived Induced Pluripotent Stem Cells.

doi: 10.5966/sctm.2014-0278

Figure Lengend Snippet: Figure 1. Evaluation of the best pair of anti-FMRP antibodies in the time-resolved fluorescence resonance energy transfer (TR-FRET) assay. (A): Confirmation of the best pair of anti-FMRP antibodies for TR- FRET-basedFMRPdetectioninacell-basedassaywithcontrolandfragile X syndrome (FXS) fibroblasts. The indicated numbers of control and FXS fibroblast cells were plated in 384-well plates, and the FMRP assay was performed. The y-axis shows the FMRP levels in control fibroblasts nor- malizedtothelevelsinFXSfibroblasts.Thenumbersonthex-axisreferto theantibodiesusedintheassayandcorrespondtotheID#asdescribedin supplemental online Table 1. The inset shows a diagrammatic represen- tation of the basic principleof TR-FRET-based FMRP assay. Two different anti-FMRP antibodies, one labeled with a donor fluorophore, europium cryptate(K),andtheotherlabeledwithanacceptor(d2),areaddedtothe cell lysate after incubation with the compounds. During detection, FRET can only occur if the two FMRP antibodies are in close proximity. This significantly reduces any nonspecific backgroundsignal thatmightresult from the two individual antibodies. (B): FMRP assay using recombinant FMRP in 384-well plates. Twofold dilutions of the recombinant FMRP protein were measured in the TR-FRET assay. The purified recombinant protein in the FMRP assay was detectable at 2 fmol/ml and was linear up to 270 fmol/ml. Abbreviations: C, control; Em, emission; Ex, excitation; FMRP,fragileXmentalretardationprotein;KC,cryptatecontrolwithonly FMRP antibody-K; NC, negative control without any antibodies.

Article Snippet: NSCs were grown in neurobasal medium supplementedwith 13B27, 13NEAA, 13GlutaMAX-1 (all from Life Technologies), and 10 ng/ml recombinant human basic fibroblast growth factor (R&D Systems, Minneapolis, MN, http:// www.rndsystems.com) on plates coated with Geltrex or CELLstart (Life Technologies).

Techniques: Fluorescence, Förster Resonance Energy Transfer, Control, FMRP Assay, Labeling, Incubation, Recombinant, Purification, Negative Control